منابع مشابه
Dilution assay statistics.
A parametric method of statistical analysis for dilution assays is developed in detail from first principles of probability and statistics. The method is based on a simple product binomial model for the experiment and produces an estimate for the concentration of target entities, a confidence interval for this concentration, and an indicator of the quality of the assay called the p value for go...
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Rapid spreading of the low pathogenic avian influenza virus (AIV) caused by the H9N2 subtype and the highly pathogenic AIV caused by H5N1 have caused serious economic losses in the poultry industries of Asia. Therefore, the early detection of AIVs is crucial for the control of the disease. In the present study, the applicability of a rapid immunochromatographic (RIC) assay, which specifically d...
متن کاملA Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein
Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...
متن کاملApplication of an end point dilution method to bacteriophage assay.
In experiments on the accumulation and elimination of virus particles by Pacific oysters (Crassostrea gigas), an Escherichia coli C2 bacteriophage was used as a virus model. It became necessary to quantitate low concentrations of phage present in samples of seawater, oyster shell liquor, and oyster meats and feces. The agar overlay phage plaque assay is subject to the same limitations with rega...
متن کاملPlate-dilution frequency technique for assay of microbial ecology.
The plate-dilution frequency technique described facilitates simultaneous enumeration of a wide range of physiologically different microorganisms in complex systems with a precision comparable to dilution tube (most probable number) methods. Replicate microsamples are inoculated from each member of a dilution series onto areas delineated on plates of prepoured solid media; the plates are incuba...
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ژورنال
عنوان ژورنال: Epidemiology and Infection
سال: 1954
ISSN: 0950-2688,1469-4409
DOI: 10.1017/s0022172400036913